| Annan | Newington Nursery Class | Hospital Rd | DG12 5 | 01461 201941 |
| Berwick-upon-Tweed | Berwick Rascals Nursery | 28, Main St Tweedmouth | TD15 2AA | 01289 303365 |
| Berwick-upon-Tweed | Spittal Day Nursery | North Greenwich Rd Spittal | TD15 1RG | 01289 330644 |
| Castle Douglas | Seabright Nursery | 15 Queens Street | DG7 1HU | 01556-502681 |
| Dumfries | ABC Nursery | Eskdale House, Crichton Royal Hospital Glencaple Rd | 01387-263337 | |
| Dumfries | Happy Faces Nursery | 25 Calside Rd | DG14HA | 01387 248944 |
| Dumfries | Catherinfield Day Nursery | Heathhall | DG1 3QZ | 01387-243839 |
| Dumfries | Georgetown Childcare Group | Georgetown Road | 01387-259622 | |
| Dumfries | Happy Faces Nursery | 25 Calside Road | DG1 4HA | 01387-248944 |
| Dumfries | Little Stars Playcare | Lincludden Comm. Centre, College Rd | 01387-248268 | |
| Dumfries | Malory House Nursery | 20 Catherine Street | DG1 1JF | 01387-269839 |
| Dumfries | Rumble Tumble | Congreational Church Hall, Irvine St | DG1 1ED | 01387-259855 |
| Duns | Never Never Land Day Nursery | 37, South St | TD11 3AB | 01361 884180 |
| Dunscore/Dumfries | Pre School Centre | Old School | DG20SY | 01387 820273 |
| Earlston | Earlston Nursery | High St | TD4 6JQ | 01896 849860 |
| Ecclefechan | ABC Nursery | Lynnecroft Road | 01576-300765 | |
| Hawick | Careshare Little Rascals Nursery | Noble Place | TD9 9QE | 01450 378051 |
| Hawick | Mansfield Nursery | Mansfield Rd | TD9 8AB | 01450 373969 |
| Hawick | Sunshine Kindergarten | Wilton Church Hall, Dickson St | TD9 7EL | 01450 370170 |
| Jedburgh | Little Treasures Nursery | 1 Market Place | 01835 864689 | |
| Kelso | Castlegate Nursery | Spylaw Road | TD5 8DN | 01573 226554 |
| Lockerbie | Elmbank Private Nursery | 41 Livingstone Place | DG11 2AU | 01576 202657 |
| Melrose | Careshare at Beeches Nursery | Huntlyburn Road | TD6 9BX | 01896 820400 |
| Melrose | Cherrytrees Children's Nursery & After School Club | Cherrytrees House, Dingleton Rd | TD6 9QS | 01896 820123 |
| Melrose | Playstation 1 | Unit G, Melrose Station Palma Place | TD6 9PR | 01896 823892 |
| Moffat | Moffat Childcare Small n Tall | Ladyknowe Hall, Ladyknowe | 01683-220460 | |
| Peebles | First Nursery Ltd | Kingsland Square | 01721 724395 | |
| Peebles | Kingsmeadows Nursery | Cavalry Park, Kingsmeadows Rd | EH45 9BU | 01721 720175 |
| Stranraer | Rephad Primary School Nursery | Aird Building Ladies Walk | DG9 8BW | 01776 703681 |
| West Linton | The Village Nursery | St. Andrews Cottage, Lower Green | EH46 7EW | 01968 661015 |
| Whithorn | Southern Machars Playcare | Community Centre, Castlehill | 01988-500902 |