| Cowdenbeath | Rascals Pre-School Nursery | 26 Hill Street | KY4 9DE | 01383-513318 |
| Cupar | Busy Bee | 56a Tannage Close | KY15 5HS | 01334 656656 |
| Cupar | Busy Bee | Elmwood College, Carslogie Rd | KY15 4HY | 01334 657111 |
| Dalgety Bay | Rainbow Nursery | 14 Moss Way Hillend Ind Park | KY11 9JS | 01383 821970 |
| Dunfermline | Bumble Beez Nursery | Eastwood House 10 Transy Place | KY12 7QN | 01383 626385 |
| Dunfermline | Careshare Nursery at Lauder College | Lauder College Halbeath Road | KY11 8JH | 01383 844640 |
| Dunfermline | Careshare Nursery at Carnegie Nursery | Castle View, Carnegie Campus | KY11 8PF | 01383 626262 |
| Dunfermline | Lilliput Lane Private Nursery | 32 Canmore Street | KY12 7NT | 01383626261 |
| Dunfermline | Little Acorns Day Nursery | 55 Beath View Touch | KY11 4UF | 01383 624409 |
| Dunfermline | Partnership in Childcare | Hillend Industrial Park Hillend | KY11 9JS | 01383 821970 |
| Dunfermline | Pitreavie Kindergarten | Pitreavie Business Park | KY11 8PU | 01383 622345 |
| Glenrothes | Careshare at Pinewood Nursery | 1 Cos Lane | KY7 4AH | 01592 750501 |
| Glenrothes | Rainbow Nursery | Saltire Centre, Pentland Park | KY6 2AL | 01592-630126 |
| Glenrothes | Sunshine Nursery Ltd | Unit 4 Newark Rd South | KY7 4NS | 01592 630485 |
| Glenrothes | Peek-a-Boo Nursery | Betson St, Markinch | KY7 6AA | 01592 760400 |
| Inverkeithing | Partnership in Childcare | Touch Centre Beith View | 01383 312830 | |
| Kinross | Careshare Nurseries at Croftbank Nursery | 30 Station Road | KY13 8TG | 01577 863819 |
| Kinross | Jack & Jills Nursery | Royal Bank House, 55 High St. | KY13 7AA | 01577 863209 |
| Kirkcaldy | Adam Smith College Nursery | Priory Campus | KY1 2QT | 01592 268591 |
| Kirkcaldy | Childsplay | 1 King Street | KY | 01592 642300 |
| Kirkcaldy | Daisychain Nursery | 144 Commercial Street | KY1 2NU | 01592 598800 |
| Kirkcaldy | Daisychain Nursery | Seaview House, 102 Loughborough Rd | KY1 3DD | 01592 653274 |
| Kirkcaldy | Kingdom Kiddies Nursery | 12 Sang Road | KY1 1EZ | 01592 643862 |
| Kirkcaldy | Sunshine Nursery Ltd | 49 Whytescauseway | KY1 1XD | 01592 641296 |
| Newport-On-Tay | Busy Bee | 30 Tay Street | DD6 8AL | 01382 541154 |
| St. Andrews | Acorn Nursery | The Steadings Kinaldy Farm | 01334 475698 | |
| St. Andrews | St. Andrews University Nursery | Fife Park Strathkinness High Street | KY16 9UA | 01334 470642 |
| St. Andrews | Wonder Years Pre-school Nursery | Albany Park East Sands | KY16 8BP | 01334 476127 |